Virus infection real-time imaging visualizes viral RNA translation within cells in real-time, revealing the earliest events of viral infection when viral protein and RNA levels are low, as well as the origins and consequences of cell-to-cell heterogeneity during virus infection.

Deformable poly-acrylamide co-acrylic acid microparticles (DAAM-particles) are tunable hydrogel microparticles for quantifying cellular forces. This comprehensive protocol details their synthesis, functionalization and applications.

Rigorous characterization is important for developing electrocatalytic reactions, for example, nitrate reduction. This workflow integrates bulk and surface techniques with in situ/operando spectroscopies, electrochemical measurements and density functional theory calculations.

The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodology to perform the assay across a range of systems.

Virus infection real-time imaging visualizes viral RNA translation within cells in real-time, revealing the earliest events of viral infection when viral protein and RNA levels are low, as well as the origins and consequences of cell-to-cell heterogeneity during virus infection.

Deformable poly-acrylamide co-acrylic acid microparticles (DAAM-particles) are tunable hydrogel microparticles for quantifying cellular forces. This comprehensive protocol details their synthesis, functionalization and applications.

This protocol integrates state-of-the-art microfabrication, tissue engineering and optogenetic approaches to generate topobiologically complex miniature colons capable of undergoing tumorigenesis in vitro.

Rigorous characterization is important for developing electrocatalytic reactions, for example, nitrate reduction. This workflow integrates bulk and surface techniques with in situ/operando spectroscopies, electrochemical measurements and density functional theory calculations.

The Mant-ATP assay is a simple and accessible means for rapid quantitative assessment of the ratio of super-relaxed myosin to disordered relaxed myosin. This protocol provides a standardized methodology to perform the assay across a range of systems.

This Protocol describes XomicsToModel, a semi-automated pipeline that integrates bibliomic, transcriptomic, proteomic and metabolomic data with a generic genome-scale metabolic reconstruction or model.

The authors present a protocol for the use of two advanced DNA-based tension probes, the reversible shearing DNA-based tension probe (RSDTP) and ForceChrono probe, which provide powerful tools for studying mechanotransduction in living cells.

This Protocol Extension describes directional conjugation of monoclonal antibodies to nanoparticles via the Fc antibody moiety using metal-free click chemistry, including procedures for synthesis, quality control and evaluation of nanoparticle–antibody conjugates.

This protocol for the use and quality control of genetically encoded reporters to label proteins and cells in correlative light and electron microscopy workflows enables the acquisition of molecular, cellular and biochemical information at high resolution.